abstract：:Myosin was isolated from pig atrial and ventricular myocardium during postnatal development and Ca2+-ATPase was determined and myosin light chains were analysed by electrophoresis in sodium dodecylsulfate polyacrylamide gel. During ontogenesis ATPase activity of ventricular myosin remains virtually unchanged, whereas ...

journal_title：General physiology and biophysics

authors： Syrový I

更新日期：1989-02-01 00:00:00

abstract：:Analogs of carbonyl cyanide phenylhydrazone providing no reaction with nucleophilic groups and lacking acidobasic properties, respectively, were synthesized for study of mechanism of uncoupling effect on oxidative phosphorylation. Their retention, influence on proton transport, abilities to SH--groups modify and to st...

journal_title：General physiology and biophysics

authors： Antalík M,Sturdík E,Sulo P,Propperová A,Mihalovová E,Podhradský D,Dzurila M

更新日期：1988-10-01 00:00:00

abstract：:The postnatal development of high-affinity 3H-muscimol binding to GABAA receptors was studied in the lateral geniculate nucleus, superior colliculus, frontal and visual cortex of the rat brain. In the lateral geniculate nucleus 3H-muscimol binding rises from day 10 through day 37 reaching the highest value during the ...

journal_title：General physiology and biophysics

authors： Schliebs R,Rothe T

更新日期：1988-06-01 00:00:00

abstract：:The dynamics of a bioreactor with a variable volume and an active solute flux based on the thermodynamics of irreversible processes and stability analysis was studied. The active solute flux may control both the bioreactor volume and the hydrostatic pressure as well as the concentration of the solute inside the cell i...

journal_title：General physiology and biophysics

authors： Wierzchaczewski M

更新日期：1988-02-01 00:00:00

abstract：:The effects of ryanodine on negative force staircase and potentiated rested-state contraction (RC) in rat myocardium were compared to the action of Ca release stimulator (caffeine) and inhibitors (local anesthetics). Only low ryanodine concentrations (0.1-0.5 mumol/l) were found to reverse anomalous mechanical pattern...

journal_title：General physiology and biophysics

authors： Saxon ME,Kobrinski EM

更新日期：1988-02-01 00:00:00

abstract：:Semiserial sections of lung capillaries of seven rabbits were examined for megakaryocytes. Triads of serial sections of each lobe were examined, and megakaryocyte counts per cm2 were determined. Median megakaryocyte count was determined for each lobe. The lobal values were used to calculate the was determined for each...

journal_title：General physiology and biophysics

authors： Resl M,Kuna P,Petýrek P

更新日期：1987-08-01 00:00:00

abstract：:Opioid mu-receptors are membrane bound receptors. The mechanism by which they transduce their biological effect into the inner compartment of the postsynaptic cell is still not fully understood. The present study was attempted to the measurement of changes of the thermodynamic parameters of the receptor--agonist/antag...

journal_title：General physiology and biophysics

authors： Zeman P,Tóth G,Kvetnanský R

更新日期：1987-06-01 00:00:00

abstract：:Various techniques have been used (UV absorbance and fluorescence spectroscopy, polarised UV fluorescence microscopy, viscosimetry, electrophoresis and isoelectric focusing, superprecipitation, etc.) As suggested by several parameters, denervation interferes with the structure of actin by producing local conformation ...

journal_title：General physiology and biophysics

authors： Strankfeld IG,Moskalenko IE

更新日期：1987-06-01 00:00:00

abstract：:Effects of six thiol reagents with different physico-chemical properties were tested on the Na+-dependent 45Ca2+ transport into the rat brain microsomal membrane vesicles. The mercurials p-chlormercuribenzoate and Mersalyl effectively inhibited 45Ca2+ uptake with IC50 values in the order of 10(-4) mol X l-1 in the med...

journal_title：General physiology and biophysics

authors： Orlický J,Ruscák M,Juhász O,Zachar J

更新日期：1987-04-01 00:00:00

abstract：:Changes in the Young elasticity modulus in perpendicular direction to the membrane surface E perpendicular, in the coefficient of dynamic viscosity eta, in the electric capacitance C, in the surface charge U1, in the conductivity g and in the coefficient of non-linearity beta of current-voltage characteristic caused b...

journal_title：General physiology and biophysics

authors： Hianik T,Zórad S,Kavecanský J,Macho L

更新日期：1987-04-01 00:00:00

abstract：:Using spin-labeled fatty acid derivatives and maleimide, the effect of temperature on the structural state of various parts of the lipid bilayer of sarcoplasmic reticulum (SR) membranes and the segmental motion of the Ca-ATPase molecule were investigated. The mobility of the spin probes localized in the hydrophobic zo...

journal_title：General physiology and biophysics

authors： Rubtsov AM,Sentjurc M,Schara M

更新日期：1986-10-01 00:00:00

abstract：:A simple graphical method is proposed to distinguish between two-state and gradual alterations in DNA conformation from circular dichroic spectra. The method is advantageous particularly to identify two-state conformational isomerizations which exhibit low degree of cooperativity. The usefulness of the method is illus...

journal_title：General physiology and biophysics

authors： Kypr J,Vorlícková M

更新日期：1986-08-01 00:00:00

abstract：:Arachidonic, linolenic and linoleic acids decreased the binding of the m-cholinergic antagonist [3H] QNB and did not affect the ratio of high to low affinity binding sites to the agonist carbamoylcholine in rat brain synaptic membranes. In the presence of arachidonic acid, SH-reagent N-ethylmaleimide acquired the abil...

journal_title：General physiology and biophysics

authors： Okun IM,Merezhinskaya NV,Rakovich AA,Volkovets TM,Aksentsev SL,Konev SV

更新日期：1986-06-01 00:00:00

abstract：:The membrane lipid and fatty acid compositions of red blood cells from a paramyotonia patient were investigated. Cholesterol and total phospholipid contents in paramyotonia were not different from control. Only the sphingomyelin content was lower, and thus the molar ratio of phosphatidylcholine/sphingomyelin was highe...

journal_title：General physiology and biophysics

authors： Szymanska G,Marx A,Melzner I,Sarzala MG,Rüdel R

更新日期：1986-04-01 00:00:00

abstract：:In the present study effects of a new local anaesthetics, pentacaine (trans-2-pyrolidinocyclohexylester of 3-pentyloxyphenylcarbamic acid), and of some chemically related compounds on rat hepatic adenylate cyclase activity were studied under various experimental conditions. As compared with tetracaine, the local anaes...

journal_title：General physiology and biophysics

authors： Hynie S,Wenke M,Klenerová V

更新日期：1985-10-01 00:00:00

abstract：:The distributions of K, Na, Mg and Ca within frog ovarian and oviductal oocytes were studied by electron probe wavelength dispersive X-ray microanalysis. An important heterogeneity could be found both in nuclear and jelly coated oocytes. The highest K, Mg and, to a lesser extent, Na concentrations were found in the pi...

journal_title：General physiology and biophysics

authors： Burovina IV,Pivovarova NB,Pogorelov AG

更新日期：1985-06-01 00:00:00

abstract：:The hyperpolarisation of the membrane potential in Characeae above that of the diffusion potential is explained by the operation of the electrogenic proton pump. We studied the interaction of calcium with the functioning of the pump. The membrane potential was measured using the standard microelectrode technique. An i...

journal_title：General physiology and biophysics

authors： Vuletić M,Vucinić Z,Radenović C

更新日期：1985-04-01 00:00:00

abstract：:Liposomes and proteoliposomes obtained from rat brain were used; structural changes induced by Vc5 cytotoxin (CT) from Central Asia cobra venom have been studied by the EPR method using spin probes (5-, 10-, or 12-doxylstearic acid). The addition of CT to liposome samples, containing spin probes resulted in the appear...

journal_title：General physiology and biophysics

authors： Aripov TF,Salakhutdinov BA,Salikhova ZT,Sadykov AS,Tashmukhamedov BA

更新日期：1984-12-01 00:00:00

abstract：:he secondary structure of membrane proteins was studied in rat heart sarcolemma by circular dichroism under conditions of interaction with metallic cofactors of (Na+ + K+)-ATPase at their optimal concentrations and under metal free conditions. Approximately 80 per cent of polypeptide chains in the membrane were organi...

journal_title：General physiology and biophysics

authors： Vrbjar N,Soós J,Ziegelhöffer A

更新日期：1984-08-01 00:00:00

abstract：:Fusion of neuraminidase or trypsin pretreated human erythrocytes and untreated cells was performed. The obtained doublets had a permanent dipole moment which disappeared as a function of time. Since the dipole moment disappearance is due to protein interdiffusion the lateral diffusion constant may be determined. This ...

journal_title：General physiology and biophysics

authors： Donath E,Arndt R

更新日期：1984-06-01 00:00:00

abstract：:The role of viscosity in coupling between chemical reaction (complex formation) and diffusion in membranes has been investigated. The Fick law was replaced by the momentum balance equation with the viscous term. The irreversible thermodynamics admits coupling of the chemical reaction rate with the gradient of velocity...

journal_title：General physiology and biophysics

authors： Gałdzicki Z,Miekisz S

更新日期：1984-04-01 00:00:00

abstract：:The fluorescent probe chlortetracycline (CTC) was used to investigate redistribution of intracellular Ca2+ in concanavalin A (Con A)-stimulated human peripheral blood lymphocytes. The addition of the mitogen to CTC-equilibrated lymphocytes induced (within 10 to 15 minutes) a Con A-concentration dependent decrease in C...

journal_title：General physiology and biophysics

authors： Gukovskaya AS,Zinchenko VP,Ryabichenko VV,Astashkin EI

更新日期：1983-10-01 00:00:00

abstract：:125I-labelled o-iodobenzoate (OIB) was prepared by means of an isotopic exchange reaction; its distribution and excretion were determined and its pharmacokinetic parameters in rats were calculated. The calculated value of the half-life of OIB elimination was 38.7 +/- 0.7 min, the distribution volume was 278.2 +/- 53.5...

journal_title：General physiology and biophysics

authors： Láznícek M,Kvĕtina J,Srámek B,Kronrád L,Hradílek P

更新日期：1983-08-01 00:00:00

abstract：:The inhibition of sodium and potassium currents in frog myelinated fibres by ajmaline (AM) and its quaternary derivative, N-propyl ajmaline (NPA), depends on voltage-clamp pulses and the state of channel gating mechanisms. The permanently charged NPA and protonated AM interact only (or mainly) with open channels, whil...

journal_title：General physiology and biophysics

authors： Khodorov BI,Zaborovskaya LD

更新日期：1983-08-01 00:00:00